What are the incubation requirements for an indirect antiglobulin test?

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Multiple Choice

What are the incubation requirements for an indirect antiglobulin test?

Explanation:
Incubation lets IgG antibodies in the serum bind to antigens on the test red cells, and this sensitization works best at body temperature. Therefore, the indirect antiglobulin test uses a 15–60 minute incubation at 37°C to allow sufficient antibody binding without unnecessary delay. Shorter times or cooler temperatures won’t give adequate sensitization, and longer times at non-physiologic temperatures aren’t needed. After this step, unbound stuff is washed away and the antiglobulin reagent is added to reveal the bound antibodies.

Incubation lets IgG antibodies in the serum bind to antigens on the test red cells, and this sensitization works best at body temperature. Therefore, the indirect antiglobulin test uses a 15–60 minute incubation at 37°C to allow sufficient antibody binding without unnecessary delay. Shorter times or cooler temperatures won’t give adequate sensitization, and longer times at non-physiologic temperatures aren’t needed. After this step, unbound stuff is washed away and the antiglobulin reagent is added to reveal the bound antibodies.

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