Which statement about LISS is not true?

• A. It increases the rate of antibody–red cell reaction
• B. It lowers ionic strength to promote agglutination
• C. It enhances the effect of albumin on Rh blood group antigens
• D. It is commonly used to enhance antibody screening

Answer: (C)

Low Ionic Strength Solution (LISS) works by reducing the ionic strength of the medium, which lowers the zeta potential on red cells. That diminished electrical repulsion lets antibodies and antigens come together more quickly, so antibody–red cell reactions happen faster and more readily. This is why LISS speeds up agglutination and is commonly used to improve antibody screening and identification in the indirect antiglobulin test.

The statement saying LISS enhances the effect of albumin on Rh antigens isn’t accurate. Albumin-based enhancement is a separate technique used to promote agglutination for certain antibodies, but LISS does not amplify albumin’s effect on Rh antigens. The other statements correctly describe LISS’s role in speeding reactions, lowering ionic strength to promote agglutination, and its use in antibody screening.